簡(jiǎn)要描述:英文名稱: Activin A,human產(chǎn)品應(yīng)用: 胚胎發(fā)育、細(xì)胞生長(zhǎng)分化中國(guó)俗稱: 激活素A 注冊(cè)品牌: StemRD 生產(chǎn)廠家: 美國(guó)StemRD公司訂購(gòu)貨號(hào): ACT-100規(guī)格型號(hào): 100?g保存溫度: ?-80℃產(chǎn)品種類: 生長(zhǎng)分化因子到貨時(shí)間: 1~5天
產(chǎn)品目錄
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產(chǎn)品英文名稱:Activin A,human
產(chǎn)品中文名稱: 人激活素A
訂購(gòu):
:gnana@hystemcell.com
Activin A,human產(chǎn)品介紹:
激活素(activins) Activin A是轉(zhuǎn)化生長(zhǎng)因子(TGF)β家族的一種分泌蛋白,可控制胚胎軸發(fā)育為有功能的前腸源性組織,調(diào)解多種細(xì)胞的生長(zhǎng)和分化。多種研究表明,其能誘導(dǎo)胰腺β細(xì) 胞的分化。尤其與其他因子的聯(lián)合作用后能發(fā)揮更顯著的誘導(dǎo)作用。如activin A聯(lián)合全反式維甲酸可以更有效地誘導(dǎo)鼠ESC為產(chǎn)胰島素細(xì)胞,而activin與BTC共同作用可以轉(zhuǎn)化新生大鼠導(dǎo)管細(xì)胞為產(chǎn)胰島素細(xì)胞。卵泡抑素 (follistatin)和表皮生長(zhǎng)因子相關(guān)肽(Cripto)是ac2tivins的天然拮抗劑,有研究表明了胰島分化階段導(dǎo)管上皮細(xì)胞里的 activins及其抑制因子follistatin和Cripto都有所增加,卵泡抑素抑制activin的同時(shí),能顯著促進(jìn)胰腺導(dǎo)管細(xì)胞新生而減少β 細(xì)胞的分化,故選擇抑制activin信號(hào)途徑可運(yùn)用于擴(kuò)增前體細(xì)胞,而激活activin有利于β細(xì)胞的分化。這些結(jié)果表說(shuō)明,胰腺生長(zhǎng)和分化之間的平 衡可通過(guò)activin信號(hào)途徑的精確調(diào)節(jié)來(lái)實(shí)現(xiàn)。同時(shí)它還被廣泛用于干細(xì)胞研究,用來(lái)誘導(dǎo)分化的胚胎干細(xì)胞形成內(nèi)胚層。
Activin A,human 產(chǎn)品特性:
? Activin A,human內(nèi)毒素含量小于0.1EU /μg,活性經(jīng)過(guò)人源細(xì)胞鑒定,純度高
? 生物活性在人源293細(xì)胞經(jīng)過(guò)SMAD實(shí)驗(yàn)鑒定The EC50為 0.5–2.0 ng/ml
? Activin A,human產(chǎn)品經(jīng)過(guò)SDS-PAGE 和HPLC雙層鑒定純度大于95%
? 只限于科學(xué)研究,非人體使用
Activin A,human 產(chǎn)品英文簡(jiǎn)述:
Activin A is a member of the TGFβ superfamily. It is a 26 kDa disulfidelinked homodimer of two βA chains, each containing 116 amino acid residues. Activin A is widely used in stem cell research. It has been used to induce the differentiation of embryonic stem cells to the definitive endoderm. The biological activities of Activin A can be neutralized by diffusible TGF-β antagonists, Follistatin (also available from StemRD). StemRD's Activin A is made in human 293 cells, which renders human-specific post-translational modifications. We purified this product through a series of chromatography including HPLC to near homogeneity.
? Purity: Greater than 95% by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a SMAD reporter gene assay in human 293 cells. The EC50 ranges from 0.5 - 2.0 ng/ml
? For Research Use Only. Not for Use in Humans
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