簡(jiǎn)要描述:英文名稱: WNT-5a, Human產(chǎn)品應(yīng)用: 信號(hào)表達(dá),胚胎發(fā)育,細(xì)胞增殖中國(guó)俗稱: 人WNT-5a 生長(zhǎng)分化因子 注冊(cè)品牌: StemRD 生產(chǎn)廠家: 美國(guó)StemRD公司訂購(gòu)貨號(hào): W5A-H-005規(guī)格型號(hào): 5?g保存溫度: ?-80℃產(chǎn)品種類: 生長(zhǎng)分化因子到貨時(shí)間: 1~5天
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WNT-5a, Human產(chǎn)品介紹:
Wnt信號(hào)轉(zhuǎn)導(dǎo)通路因其啟動(dòng)蛋白為Wnt蛋白而得名,在胚胎發(fā)育、細(xì)胞增殖及分化等方面發(fā)揮重要 作用,其異?;罨c人類多種腫瘤的發(fā)生密切相關(guān)。研究顯示,Wnt信號(hào)至少存在經(jīng)典和非經(jīng)典兩條不同的信號(hào)通路,而Wnt-5a屬于非經(jīng)典Wnt信號(hào)通 路,既往研究Ror2具有Wnt受體結(jié)構(gòu)域樣結(jié)構(gòu)。有研究認(rèn)為Wnt-5a經(jīng)由Ror2介導(dǎo)的 信號(hào)通路發(fā)揮作用。
Human WNT-5a蛋白含有357個(gè)氨基酸殘基。
WNT-5a, Human 產(chǎn)品特性:
? 活性在共轉(zhuǎn)Frizzled- 4 和 LRP- 5基因的HEK293 細(xì)胞鑒定,純度高,無動(dòng)物成分污染
? WNT-5a, Human活性在共轉(zhuǎn)Frizzled- 4 和 LRP- 5基因的HEK293 細(xì)胞鑒定
? WNT-5a, Human產(chǎn)品經(jīng)過SDS-PAGE 和HPLC雙層鑒定純度大于85%
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WNT-5a, Human產(chǎn)品英文簡(jiǎn)述:
WNT-5a belongs to the class of WNT proteins that activate the “noncanonical" pathway. The predicted size of human WNT-5a is a monomeric protein containing 357 amino acid residues. Due to glycosylation, it migrates at an apparent molecular weight of ~45 kDa on SDS-PAGE under non-reducing conditions. StemRD’s product is expressed from a human cell line in animal-free medium, and purified with a proprietary process that is distinct from the published method.
? Purity: Greater than 85% as determined by SDS-PAGE and HPLC analysis
? Biological Activity: The activity was determined by using a TCF reporter gene assay in HEK293 cells co-transfected with Frizzled-4 and LRP- 5. WNT-5a activates (instead of inhibits) the TCF reporter gene in this assay (MilkelsAJ, et al., PLoS Biol, 4:e115, 2006). This activation mode is utilized because activation assays are generally more reliable than inhibition assays, as they are less prone to any non-specific inhibitory contamination in the preparation.